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nitrocefin solution (100 μg/ml)  (Millipore)


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    Millipore nitrocefin solution (100 μg/ml)
    Nitrocefin Solution (100 μg/Ml), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitrocefin+solution/nitrocefin+solution/pmc02824592-244-20-34
    Average 90 stars, based on 1 article reviews
    nitrocefin solution (100 μg/ml) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Pandemic serotypes of Vibrio cholerae isolated from ships' ballast tanks and coastal waters: assessment of antibiotic resistance and virulence genes (tcpA and ctxA).
    Article Snippet: There is concern that ships’ ballasting operations may disseminate Vibrio cholerae to ports throughout the world.. Given evidence that the bacterium is indeed transported by ships, we isolated pandemic serotypes O1 and O139 from ballast tanks and characterized them with respect to antibiotic resistance and virulence genes ctxA and tcpA.. We carried out concurrent studies with V. cholerae isolated from coastal waters.

    Purification:

    Article Title: Resistance to Cefepime and Cefpirome Due to a 4-Amino-Acid Deletion in the Chromosome-Encoded AmpC β-Lactamase of a Serratia marcescens Clinical Isolate
    Article Snippet: .. The -lactamase extracts from cultures of clinical isolates and purified enzymes were subjected to analytical isoelectric focusing (IEF) analysis as previously described (3) by using an ampholine polyacrylamide gel with a pH range of 3.5 to 9.5 (ampholine PAG plate; Amersham Pharmacia Biotech) for 90 min at 1,500 V, 50 mA, and 30 W. The focused -lactamases were detected by overlaying the gel with a 1 mM nitrocefin solution (Calbiochem, Merck Eurolab SAS, Fontenay-sous-bois, France). ..

    Article Title: TEM-121, a Novel Complex Mutant of TEM-Type ?-Lactamase from Enterobacter aerogenes
    Article Snippet: .. The β-lactamase extracts from cultures of clinical isolates and purified enzymes were subjected to analytical isoelectric focusing (IEF) as previously described ( 4 ), using an ampholine polyacrylamide gel with a pH range of 3.5 to 9.5 (ampholine polyacrylamide gel plate; Amersham Pharmacia Biotech) for 90 min at 1,500 V, 50 mA, and 30 W. The focused β-lactamases were detected by overlaying the gel with a 1 mM nitrocefin solution (Calbiochem, Merck Eurolab SAS, Fontenay-sous-bois, France). ..

    Article Title: Resistance to Cefepime and Cefpirome Due to a 4-Amino-Acid Deletion in the Chromosome-Encoded AmpC ?-Lactamase of a Serratia marcescens Clinical Isolate
    Article Snippet: .. IEF analysis The β-lactamase extracts from cultures of clinical isolates and purified enzymes were subjected to analytical isoelectric focusing (IEF) analysis as previously described ( 3 ) by using an ampholine polyacrylamide gel with a pH range of 3.5 to 9.5 (ampholine PAG plate; Amersham Pharmacia Biotech) for 90 min at 1,500 V, 50 mA, and 30 W. The focused β-lactamases were detected by overlaying the gel with a 1 mM nitrocefin solution (Calbiochem, Merck Eurolab SAS, Fontenay-sous-bois, France). ..

    Article Title: TEM-121, a Novel Complex Mutant of TEM-Type β-Lactamase from Enterobacter aerogenes
    Article Snippet: .. The -lactamase extracts from cultures of clinical isolates and purified enzymes were subjected to analytical isoelectric focusing (IEF) as previously described (4), using an ampholine polyacrylamide gel with a pH range of 3.5 to 9.5 (ampholine polyacrylamide gel plate; Amersham Pharmacia Biotech) for 90 min at 1,500 V, 50 mA, and 30 W. The focused -lactamases were detected by overlaying the gel with a 1 mM nitrocefin solution (Calbiochem, Merck Eurolab SAS, Fontenay-sous-bois, France). ..

    Article Title: Genetic and biochemical characterization of the chromosome-encoded class B beta-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1).
    Article Snippet: Results: Metallo-b-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B b-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively).. SLB-1 had an EDTA-inhibited and broad-spectrum b-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid.

    Electrofocusing:

    Article Title: Resistance to Cefepime and Cefpirome Due to a 4-Amino-Acid Deletion in the Chromosome-Encoded AmpC β-Lactamase of a Serratia marcescens Clinical Isolate
    Article Snippet: .. The -lactamase extracts from cultures of clinical isolates and purified enzymes were subjected to analytical isoelectric focusing (IEF) analysis as previously described (3) by using an ampholine polyacrylamide gel with a pH range of 3.5 to 9.5 (ampholine PAG plate; Amersham Pharmacia Biotech) for 90 min at 1,500 V, 50 mA, and 30 W. The focused -lactamases were detected by overlaying the gel with a 1 mM nitrocefin solution (Calbiochem, Merck Eurolab SAS, Fontenay-sous-bois, France). ..

    Article Title: TEM-121, a Novel Complex Mutant of TEM-Type ?-Lactamase from Enterobacter aerogenes
    Article Snippet: .. The β-lactamase extracts from cultures of clinical isolates and purified enzymes were subjected to analytical isoelectric focusing (IEF) as previously described ( 4 ), using an ampholine polyacrylamide gel with a pH range of 3.5 to 9.5 (ampholine polyacrylamide gel plate; Amersham Pharmacia Biotech) for 90 min at 1,500 V, 50 mA, and 30 W. The focused β-lactamases were detected by overlaying the gel with a 1 mM nitrocefin solution (Calbiochem, Merck Eurolab SAS, Fontenay-sous-bois, France). ..

    Article Title: Resistance to Cefepime and Cefpirome Due to a 4-Amino-Acid Deletion in the Chromosome-Encoded AmpC ?-Lactamase of a Serratia marcescens Clinical Isolate
    Article Snippet: .. IEF analysis The β-lactamase extracts from cultures of clinical isolates and purified enzymes were subjected to analytical isoelectric focusing (IEF) analysis as previously described ( 3 ) by using an ampholine polyacrylamide gel with a pH range of 3.5 to 9.5 (ampholine PAG plate; Amersham Pharmacia Biotech) for 90 min at 1,500 V, 50 mA, and 30 W. The focused β-lactamases were detected by overlaying the gel with a 1 mM nitrocefin solution (Calbiochem, Merck Eurolab SAS, Fontenay-sous-bois, France). ..

    Article Title: TEM-121, a Novel Complex Mutant of TEM-Type β-Lactamase from Enterobacter aerogenes
    Article Snippet: .. The -lactamase extracts from cultures of clinical isolates and purified enzymes were subjected to analytical isoelectric focusing (IEF) as previously described (4), using an ampholine polyacrylamide gel with a pH range of 3.5 to 9.5 (ampholine polyacrylamide gel plate; Amersham Pharmacia Biotech) for 90 min at 1,500 V, 50 mA, and 30 W. The focused -lactamases were detected by overlaying the gel with a 1 mM nitrocefin solution (Calbiochem, Merck Eurolab SAS, Fontenay-sous-bois, France). ..

    other:

    Article Title: Emergence of a cefepime- and cefpirome-resistant Citrobacter freundii clinical isolate harbouring a novel chromosomally encoded AmpC beta-lactamase, CMY-37.
    Article Snippet: Citrobacter freundii strain 4306 was isolated from a urine specimen of a patient in March 2006 in Palestine.. This strain showed unique multidrug resistance phenotype, as it was resistant both to 7-methoxyand oxyimino-cephalosporins, including cefepime, efpirome and monobactams, in addition to quinolones, streptomycin and trimethoprim/sulfamethoxazole.. Clavulanic acid did not act synrgistically with cephalosporins by the double-disk synergy test.

    Recombinant:

    Article Title: Genetic and biochemical characterization of the chromosome-encoded class B beta-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1).
    Article Snippet: Results: Metallo-b-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B b-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively).. SLB-1 had an EDTA-inhibited and broad-spectrum b-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid.

    Plasmid Preparation:

    Article Title: Genetic and biochemical characterization of the chromosome-encoded class B beta-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1).
    Article Snippet: Results: Metallo-b-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B b-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively).. SLB-1 had an EDTA-inhibited and broad-spectrum b-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid.



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    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of <t>nitrocefin</t> degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
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    Image Search Results


    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of nitrocefin degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .

    Journal: Nature Communications

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae

    doi: 10.1038/s41467-024-47708-4

    Figure Lengend Snippet: Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of nitrocefin degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .

    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Techniques: Staining, Cryo-EM Sample Prep, Membrane, Permeability, Incubation, Positive Control, Standard Deviation, Concentration Assay